optical adhesive film microamptm (Thermo Fisher)
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optical adhesive film microamptm
Optical Adhesive Film Microamptm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microamptm+optical+adhesive+film/microamp+optical+adhesive+film/pm40561143-100-12-16
Average 90 stars, based on 1 article reviews
Optical Adhesive Film Microamptm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microamptm+optical+adhesive+film/microamp+optical+adhesive+film/pm40561143-100-12-16
Average 90 stars, based on 1 article reviews
optical adhesive film microamptm - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: Increased Expression of miR-223-3p and miR-375-3p and Anti-Inflammatory Activity in HDL of Newly Diagnosed Women in Advanced Stages of Breast Cancer. Article Snippet: The plate was then sealed with Adhesive:Article Title: Protein Compartments Modulate Fibrillar Self-Assembly. Article Snippet: .. The plate was sealed with Article Title: Crystal structures of DCAF1-PROTAC-WDR5 ternary complexes provide insight into DCAF1 substrate specificity. Article Snippet: .. Reactions were aliquoted into 20 μL per well in a MicroAmpTM EnduraPlateTM Optical 384-well plate (Applied Biosystems, Cat# A36931) and sealed with Article Title: Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 ® Advanced XL System. Article Snippet: .. • SERATEC® PMB Test Kit (SERATEC GmbH, Göttingen, Germany); • SERATEC® PSA Semiquant Kit (SERATEC GmbH, Göttingen, Germany); • EZ1&2® Advanced XL DNA Investigator® Kit (QIAGEN, Hilden, Germany); • 1 M Dithiothreitol Solution (DTT) (Thermo Fisher Scientific, Waltham, MA, USA); • Sterile pipette tips (2.5 μL, 10 μL, 20 μL, 100 μL, 200 μL, and/or 1000 μL) (Neptune Scientific, San Diego, CA, USA); • 2 mL microcentrifuge tubes (Eppendorf, Hamberg, Germany); • 0.2 mL PCR amplification tubes (Bio Basic Inc., Markham, ON, Canada); • Kimwipes® (Kimberly-Clark Professional, Kimtech, Roswell, GA, USA); • Spin baskets (Eppendorf, Hamberg, Germany); • Conical tubes (5 mL, 15 mL, or 50 mL) (Corning, Corning, NY, USA); • Extraction Buffer (1 M Tris-HCL, 0.5 M EDTA, 20% SDS, Sodium Chloride); • TE Buffer (1 M Tris-HCL, 0.5 M EDTA); • QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific, Waltham, MA, USA); • MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, Waltham, MA, USA); Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Article Title: The WalKR two-component regulator coordinates cell wall homeostasis with DNA replication in Staphylococcus aureus Article Snippet: .. The plates were sealed with Article Title: Bisphosphonate-Modified Serum Albumin as an Effective Inhibitor of Calciprotein Particle Maturation with Long Plasma Retention Time Article Snippet: Chronic kidney disease (CKD) has recently become one of the leading causes of death and this disease is associated with vascular calcification (VC) which is caused by the deposition of calcium phosphate minerals on the intimal layer of blood vessels [1–3].. The mechanism of the mineral deposition involves calciprotein particles (CPPs), which are formed from calcium phosphate minerals and a mineral-binding blood protein fetuin-A.. CPPs are categorized into two types, an amorphous type called primary CPP (CPPI), and an insoluble crystalline type called secondary CPP (CPPII) [4]. Article Title: Unguarded liabilities: Borrelia burgdorferi’s complex amino acid dependence exposes unique avenues of inhibition Article Snippet: .. A total reaction volume of 20 μL was loaded in triplicate in MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, USA) sealed with Incubation:Article Title: Protein Compartments Modulate Fibrillar Self-Assembly. Article Snippet: .. The plate was sealed with Article Title: The WalKR two-component regulator coordinates cell wall homeostasis with DNA replication in Staphylococcus aureus Article Snippet: .. The plates were sealed with Fluorescence:Article Title: Protein Compartments Modulate Fibrillar Self-Assembly. Article Snippet: .. The plate was sealed with Software:Article Title: Protein Compartments Modulate Fibrillar Self-Assembly. Article Snippet: .. The plate was sealed with Article Title: Unguarded liabilities: Borrelia burgdorferi’s complex amino acid dependence exposes unique avenues of inhibition Article Snippet: .. A total reaction volume of 20 μL was loaded in triplicate in MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, USA) sealed with Sterility:Article Title: Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 ® Advanced XL System. Article Snippet: .. • SERATEC® PMB Test Kit (SERATEC GmbH, Göttingen, Germany); • SERATEC® PSA Semiquant Kit (SERATEC GmbH, Göttingen, Germany); • EZ1&2® Advanced XL DNA Investigator® Kit (QIAGEN, Hilden, Germany); • 1 M Dithiothreitol Solution (DTT) (Thermo Fisher Scientific, Waltham, MA, USA); • Sterile pipette tips (2.5 μL, 10 μL, 20 μL, 100 μL, 200 μL, and/or 1000 μL) (Neptune Scientific, San Diego, CA, USA); • 2 mL microcentrifuge tubes (Eppendorf, Hamberg, Germany); • 0.2 mL PCR amplification tubes (Bio Basic Inc., Markham, ON, Canada); • Kimwipes® (Kimberly-Clark Professional, Kimtech, Roswell, GA, USA); • Spin baskets (Eppendorf, Hamberg, Germany); • Conical tubes (5 mL, 15 mL, or 50 mL) (Corning, Corning, NY, USA); • Extraction Buffer (1 M Tris-HCL, 0.5 M EDTA, 20% SDS, Sodium Chloride); • TE Buffer (1 M Tris-HCL, 0.5 M EDTA); • QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific, Waltham, MA, USA); • MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, Waltham, MA, USA); Transferring:Article Title: Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 ® Advanced XL System. Article Snippet: .. • SERATEC® PMB Test Kit (SERATEC GmbH, Göttingen, Germany); • SERATEC® PSA Semiquant Kit (SERATEC GmbH, Göttingen, Germany); • EZ1&2® Advanced XL DNA Investigator® Kit (QIAGEN, Hilden, Germany); • 1 M Dithiothreitol Solution (DTT) (Thermo Fisher Scientific, Waltham, MA, USA); • Sterile pipette tips (2.5 μL, 10 μL, 20 μL, 100 μL, 200 μL, and/or 1000 μL) (Neptune Scientific, San Diego, CA, USA); • 2 mL microcentrifuge tubes (Eppendorf, Hamberg, Germany); • 0.2 mL PCR amplification tubes (Bio Basic Inc., Markham, ON, Canada); • Kimwipes® (Kimberly-Clark Professional, Kimtech, Roswell, GA, USA); • Spin baskets (Eppendorf, Hamberg, Germany); • Conical tubes (5 mL, 15 mL, or 50 mL) (Corning, Corning, NY, USA); • Extraction Buffer (1 M Tris-HCL, 0.5 M EDTA, 20% SDS, Sodium Chloride); • TE Buffer (1 M Tris-HCL, 0.5 M EDTA); • QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific, Waltham, MA, USA); • MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, Waltham, MA, USA); Polymerase Chain Reaction:Article Title: Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 ® Advanced XL System. Article Snippet: .. • SERATEC® PMB Test Kit (SERATEC GmbH, Göttingen, Germany); • SERATEC® PSA Semiquant Kit (SERATEC GmbH, Göttingen, Germany); • EZ1&2® Advanced XL DNA Investigator® Kit (QIAGEN, Hilden, Germany); • 1 M Dithiothreitol Solution (DTT) (Thermo Fisher Scientific, Waltham, MA, USA); • Sterile pipette tips (2.5 μL, 10 μL, 20 μL, 100 μL, 200 μL, and/or 1000 μL) (Neptune Scientific, San Diego, CA, USA); • 2 mL microcentrifuge tubes (Eppendorf, Hamberg, Germany); • 0.2 mL PCR amplification tubes (Bio Basic Inc., Markham, ON, Canada); • Kimwipes® (Kimberly-Clark Professional, Kimtech, Roswell, GA, USA); • Spin baskets (Eppendorf, Hamberg, Germany); • Conical tubes (5 mL, 15 mL, or 50 mL) (Corning, Corning, NY, USA); • Extraction Buffer (1 M Tris-HCL, 0.5 M EDTA, 20% SDS, Sodium Chloride); • TE Buffer (1 M Tris-HCL, 0.5 M EDTA); • QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific, Waltham, MA, USA); • MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, Waltham, MA, USA); Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Amplification:Article Title: Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 ® Advanced XL System. Article Snippet: .. • SERATEC® PMB Test Kit (SERATEC GmbH, Göttingen, Germany); • SERATEC® PSA Semiquant Kit (SERATEC GmbH, Göttingen, Germany); • EZ1&2® Advanced XL DNA Investigator® Kit (QIAGEN, Hilden, Germany); • 1 M Dithiothreitol Solution (DTT) (Thermo Fisher Scientific, Waltham, MA, USA); • Sterile pipette tips (2.5 μL, 10 μL, 20 μL, 100 μL, 200 μL, and/or 1000 μL) (Neptune Scientific, San Diego, CA, USA); • 2 mL microcentrifuge tubes (Eppendorf, Hamberg, Germany); • 0.2 mL PCR amplification tubes (Bio Basic Inc., Markham, ON, Canada); • Kimwipes® (Kimberly-Clark Professional, Kimtech, Roswell, GA, USA); • Spin baskets (Eppendorf, Hamberg, Germany); • Conical tubes (5 mL, 15 mL, or 50 mL) (Corning, Corning, NY, USA); • Extraction Buffer (1 M Tris-HCL, 0.5 M EDTA, 20% SDS, Sodium Chloride); • TE Buffer (1 M Tris-HCL, 0.5 M EDTA); • QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific, Waltham, MA, USA); • MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, Waltham, MA, USA); Extraction:Article Title: Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 ® Advanced XL System. Article Snippet: .. • SERATEC® PMB Test Kit (SERATEC GmbH, Göttingen, Germany); • SERATEC® PSA Semiquant Kit (SERATEC GmbH, Göttingen, Germany); • EZ1&2® Advanced XL DNA Investigator® Kit (QIAGEN, Hilden, Germany); • 1 M Dithiothreitol Solution (DTT) (Thermo Fisher Scientific, Waltham, MA, USA); • Sterile pipette tips (2.5 μL, 10 μL, 20 μL, 100 μL, 200 μL, and/or 1000 μL) (Neptune Scientific, San Diego, CA, USA); • 2 mL microcentrifuge tubes (Eppendorf, Hamberg, Germany); • 0.2 mL PCR amplification tubes (Bio Basic Inc., Markham, ON, Canada); • Kimwipes® (Kimberly-Clark Professional, Kimtech, Roswell, GA, USA); • Spin baskets (Eppendorf, Hamberg, Germany); • Conical tubes (5 mL, 15 mL, or 50 mL) (Corning, Corning, NY, USA); • Extraction Buffer (1 M Tris-HCL, 0.5 M EDTA, 20% SDS, Sodium Chloride); • TE Buffer (1 M Tris-HCL, 0.5 M EDTA); • QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific, Waltham, MA, USA); • MicroAmpTM Optical 96-Well Reaction Plate (Thermo Fisher Scientific, Waltham, MA, USA); Real-time Polymerase Chain Reaction:Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Mass Measurement:Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Western Blot:Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Imaging:Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Spectrophotometry:Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Nucleic Acid Electrophoresis:Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies Microscopy:Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression. Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies |